Effect of Light Intensity on the Release and Attachment of Gracilaria sp. Spores

Sri Wahidah; Andi Puspa Sari Idris; Mohamad Adnan Baiduri

doi:10.55927/d98tqh86

Authors

Sri Wahidah Department of Aquaculture Technology, State Agricultural Polytechnic of Pangkajene Islands Author
Andi Puspa Sari Idris Department of Aquaculture Technology, State Agricultural Polytechnic of Pangkajene Islands Author
Mohamad Adnan Baiduri Department of Aquaculture Technology, State Agricultural Polytechnic of Pangkajene Islands Author

DOI:

https://doi.org/10.55927/d98tqh86

Keywords:

Gracilaria Sp., Spore Culture, Light Intensity, Spore Release, Spore Attachment

Abstract

The availability of high-quality seedlings remains a major constraint in the sustainable expansion of Gracilaria aquaculture, particularly when vegetative propagation dominates seed supply. Spore culture offers an alternative route for producing generative seedlings, but its success depends strongly on environmental conditions during the early reproductive phase. This study evaluated the effect of three light intensities on the release and attachment of Gracilaria sp. spores under laboratory conditions. A completely randomized design was applied with three treatments, namely 500 lux, 1000 lux, and 1500 lux, each with three replicates. Fertile thalli in the carpospore stage were acclimatized, sterilized, and cultured in sterile seawater enriched with Grund medium under an 8 h light:16 h dark photoperiod at 25–27°C. Spore release was observed using a Sedgwick–Rafter chamber, whereas attached spores were counted under a stereo microscope. Light intensity significantly affected both response variables (one-way ANOVA, P < 0.05). The 1000 lux treatment produced the highest spore release, reaching 720 ± 35 spores/cystocarp on day 4, with the highest mean release of 452 spores/cystocarp. The same treatment also yielded the highest spore attachment, peaking at 520 ± 49 spores/cystocarp on day 3, with a mean of 391 spores/cystocarp. These findings indicate that moderate light intensity provides the most favorable condition for early reproductive performance in Gracilaria sp. spore culture and may support the development of more reliable seedling production systems for seaweed aquaculture

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